In vitro checkerboard data correlate with in vivo outcome of polyene+azole combination therapy against experimental candidiasis

A. Elefanti*, M. Siopi, P. Verweij, J.W. Mouton, J. Meletiadis

Author address: 

(Athens, GR; Nijmegen, NL)

Abstract: 

Objectives: In vivo/in vitro correlation of antifungal combination testing is necessary in order to reliably assess the efficacy of combination regimens. We therefore attempted to correlate in vitro checkerboard testing of posaconazole (POS) and amphotericin B (AMB) with the in vivo outcome of combination therapy against experimental candidiasis in a neutropenic murine model. Methods: For, the in vitro experiments, serial twofold drug dilutions were prepared in 96-well microtitration plates at concentrations of 0.008-0.5 mg/L for AMB and 0.00006-0.06 mg/L for POS alone and in combination (8 · 12 checkerboard) in quadruplicate. After inoculation with 2.5 · 103 CFU/mL of a Candida albicans clinical isolate, plates were incubated for 48 hours at 37C and read spectrophotometrically at 405 nm. For the in vivo experiments 4- 6 weeks old CD1 female mice were rendered neutropenic before i.v. infection with 105 CFU/mouse of the C. albicans isolate. In vivo 3 · 3
2012

abstract No: 

NULL

Full conference title: 

22nd European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 22nd (2012)