Infection with Cryptococcus neoformans is an increasing problem in immunocompromised patients, particularly those with acquired immune deficiency syndrome. A total of nine Cryptococcus neoformans strains were isolated from cerebrospinal fluid (CSF) of hospitalized patients with AIDS, from the Hospital Jõao Alves Filho, Aracaju, SE, Brazil. The isolates were tested for their susceptibility to amphotericin B, 5-flucytosine, terbinafine, fluconazole and itraconazole. One standard strain of C. neoformans (ATCC 32608) was included in this study. The tests were performed according to the National Committee of Clinical Laboratory Standards (NCCLS) recommendations. The microdilution method was performed with 10 3 CFU ml -1 and the incubation was at 35••••C for 72h. There was some variation in the MIC values for the five tested antifungal drugs. The MIC values ranged from 125.0 to 0.4875 µg/ml -1 for amphotericin B, from 125.0 to 0.243 µg/ml -1 for terbinafine, from 3,91 to 125 µg/ml -1 for fluconazole, from 125 to 0.243 µg/ml -1 for itraconazole and from 0.975 to 250 µg/ml -1 for 5-flucytosine. Our results have indicated a high level of resistance to all the tested antifungal drugs and may reflect the recent widespread use of antifungal drugs for long-term maintenance of patients with AIDS-associated cryptococcal meningitis.
Full conference title:
The 15 th Congress of the International Society for Human and Animal Mycology
- ISHAM 15th (2003)