In Vitro Activity of HMR 3270 against Yeasts and Filamentous Fungi

F. DROMER, M. ABDOUL, L. IMPROVISI, B. DUPONT

Author address: 

Institut Pasteur, Paris, France.

Abstract: 

Background: In vitro activity of HMR 3270 a new peptide antifungal agent of the candin class was tested in comparison with fluconazole (FCZ), itraconazole (ITZ) and amphotericin B (AM.B). Material and methods: 248 yeasts strains isolated from clinical samples were tested according to a microtiter modification of the NCCLS M 27A method (difference in the final inoculum ~ 104/well). The NCCLS method was adapted to test 98 sporulating filamentous fungi. MIC (mg/L) was the lowest drug concentration that reduced growth by 80% (100% for AM.B). Results: The table indicates the MIC90 after 48h incubation for Candida spp and 72h for other species. C.alb S-FCZ n=30 C.alb R-FCZ n=33 C. gla n=30 C. kru n=29 C. kef n=10 C. lus n=10 C. pa n=31 C.tro n=32 C. neof n=30 Trichos n=13 HMR 0.19 0.5 1 4 1.3 4 4 2 8.8 8 FCZ 4 ³64 ³64 ³64 ³64 35.2 8 ³64 8.8 8 ITZ ³8 ³8 ³8 1.2 0.9 0.9 0.125 ³8 0.5 1 AM.B 1 1 1 2 2 1.1 1 2 0.5 1 Against filamentous fungi: Aspergillus spp (n=50), Alternaria spp (n=6), Fusarium spp (n=12), Scedosporium apiospermum (n=20), S. inflatum (n=10), the ranges of MIC after 72h of incubation were 0.25 to ³ 64 mg/L for HMR 3270, 0.015 to ³ 8 mg/L for ITZ, 0.06 to ³ 8 for AM.B and ³ 64 mg/L for FCZ. Conclusion: HMR 3270 is a highly active compound against Candida. Promising preliminary results obtained in a model of systemic murine aspergillosis indicate that in vitro results against filamentous fungi may be test dependent. HMR 3270 deserves further in vitro and in vivo studies.
2001

abstract No: 

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Full conference title: 

ICAAC 41st
    • ICAAC 41st