Treatment for fungal disease is limited to a small number of antifungal drugs. Increase resistance of the Aspergillus species towards antifungal compounds; claim the search of new therapeutic alternative. Among medical herb species to have that which is show antifungal activity drugs? The objective of this study was to examine in the vitro susceptibility and antifungal activity of Aspergillums species to Syzygium aromaticum, in 60% ethyl alcohol extracts. Develop of new medical herbs agents against Aspergillums species.
Aspergillums species were isolated from different hospitals patient samples. In this, experimental works use this Aspergillums species: A. niger; A. fumigates; A.flavus; A.terreus; A. clavatus; A. glaucus and A. nidulans. Syzygium aromaticum extracted by 60% ethyl alcohol in concentrations of 5g/50 ml (50mg/ml.). On the Mueller Hinton agar inoculated with Aspergillus species and after inoculation made in the agar 10mm in diameter wells. Extract of 50mg/ml Syzygium aromaticum used directly and intoeach well-added (50 to 100 μl). In one, well add 60% ethyl alcohol without Syzygium aromaticum served as control. Plates incubated between 18/36 hour at 24°C.
Zone of inhibition made in the mm. The highest of the alcohol extracts dilution, which incorporated into well on agar medium, and showing no visible growth Aspergillums species after incubate, regarded and referred to as the minimum inhibitory concentration or MIC. The zone of inhibition test shows varies among different Aspergillums species. All Aspergillums species that where isolated clinically from patients is 100% sensitivity towards tested of Syzygium aromaticum.
The findings of this study showed that Syzygium aromaticum have anti-fungal valid alternative function against Aspergillums species. Therefore, they can be use in pharmacology. The in vitro sensitive of various Aspergillums species suggest the possibility of making pharmaceuticals preparations, medical products to suppress infection, priority of the skin (soap, creams, curative water, oil preservative) and mucosal surface (have tea, dentifrice and cavity washings) caused by Aspergillums species.
- AAA 7th (2016)