In Vitro Activity of Anidulafungin and Caspofungin in Candida Species: There is a Significant Correlation Between the Activity of Both Candins?

R. INSA, T. PELíEZ, M. GOYANES, L. ALCALA, J. MARTINEZ-ALARCON, J. GUINEA, E. BOUZA;

Author address: 

Hosp. Gen. Universitario Gregorio Marañon, Madrid, Spain.

Abstract: 

Background: Anidulafungin (AN) and caspofungin (CS) are new antifungal agents belonging to the group of candins. The aim of this study is to evaluate the in vitro activity of AN and CS against 230 selected clinical isolates of Candida spp. in our hospital. Methods: We selected a representative set of 230 Candida isolates. Antifungal susceptibility testing was performed by the E-test and by broth microdilution methods (CLSI, M27-A2), and we used a 24-h prominent inhibition end-point to determine the MIC (MIC-1, defined as ≥ 80% inhibition relative to control growth). The correlation between the activities of both candins was calculated using Pearson’s correlation coefficient. Results: Of 230 isolates, species distribution was: 61 C. albicans, 114 C. parapsilosis, 16 C. tropicalis, 17 C. glabrata, 6 C. krusei, 5 C. lusitaniae, 3 C. catenulata and 8 other Candida spp. AN was very active against Candida spp. Results (expressed as MIC90 / geometric mean MIC) were: 2/ 0.197 μg/ml. The range of MICs was 0.002-256 μg/ml, and 91.7% of the isolates were inhibited at an MIC of 8804; 2 μg/ml. Of the isolates with AN MICs of >2 μg/ml, 18 isolates were C. parapsilosis, and 1 C. tropicalis. The values of MIC90 / geometric mean MIC for CS were: 2/ 0.219 μg/ml. The range of MICs was 0.006-64 μg/ml, and 91.3% of the isolates were inhibited at a MIC of 8804; 2 μg/ml. Of the isolates with CS MICs of >2 μg/ml, 15 isolates were C. parapsilosis, 2 C. guilliermondii, 2 C. catenulata and 1 C. tropicalis. Overall geometric mean MIC (μg/ml) for all the Candida spp. tested was 0.197 for AN and 0.219 for CS. We obtained a Pearson correlation coefficient=0.633 (p
2006

abstract No: 

M-1592

Full conference title: 

46th Interscience Conference on Antimicrobial Agents and Chemotherapy
    • ICAAC 46th