In vitro activity of 8 antifungal drugs against 126 environmental and clinical isolates of Aureobasidium pullulans

M. J. Najafzadeh, G. S. de Hoog, M. Saradeghi Keisari, C. H., Klaassen and J. Meis;


Objectives: Aureobasidium pullulans is a ubiquitous black yeast-like fungus colonizing smooth surfaces of plant leaves, glass and rock, and may contaminate metal, glassware and tubing systems in the hospital. Occasionally opportunistic infections are noted in skin or on nails. In severely compromised patients deep infections are encountered, and the fungus can be isolated from blood, BAL or lymph.
Methods: The antifungal activities of eight antifungal drugs were evaluated against 126 clinical and environmental isolates of Aureobasidium pullulans. In vitro susceptibilities were determined as described in CLSI document M 38-A2.
Results: Drugs tested, including their range, were: £0.016- 16 lg ml-1 for amphotericin B (AMB), 4 - ‡64 lg ml-1 for fluconazole (FLU), £0.016-2 lg ml-1 for itraconazole (ITZ), £0.016-16 for voriconazole (VOR), 0.016-4 lg ml-1 for posaconazole (POS), 0.016- 8 lg ml-1 for Isavuconazole (ISA), 0.063-8 lg ml-1 for caspofungin (CAS) and £0.0008-8 lg ml-1 for micafungin (MICA). The resulting MIC 90s for all strains were as follows, in increasing order: posaconazole 0.25 lg ml-1, itraconazole 0.5 lg ml-1, amphotericin B 1 lg ml-1, micafungin and voriconazole 2 lg ml-1; Isavuconazole & caspofungin 4 lg ml-1, and fluconazole ‡ 64 lg ml-1.
Conclusion: The fungus shows high degrees of susceptibility to all commonly used antifungals. Most active drugs against A. pullulans are: posaconazole, itraconazole, amphotericinB, micafungin and voriconazole.

abstract No: 

    • ISHAM 18th (2012)