In vitro activities of nine antifungal drugs against 81 isolates of cutaneous fungi in Phialophora and Cyphellophora

P. Feng, M. J. Najafzadeh, J. Sun, S. Abdalla Ahmed, L. Xi, I. Curfs-Breuker, J. F. Meis and G. S. de Hoog


The in vitro activities of nine antifungal drugs against isolates of Cyphellophora laciniata (n = 3), C.vermispora (n = 3), C. pluriseptata (n = 3), C. suttonii (n = 3), C. guyanensis (n = 15), Phialophora oxyspora (n = 3), P. reptans (n = 8) and P. europaea (n = 43) were tested. The species concerned regularly are found on human skin and nails. Compounds tested (fluconazole, itraconazole, voriconazole, posaconazole, Isavuconazole, caspofungin, micafungin, amphotericin B, and terbinafin) were processed by a broth microdilution technique adaptation from the National Committee for Clinical Laboratory Standards M-38A reference method. MICs and MECs were determined spectrophotometrically and by visual inspection comparing the growth in the 96-wells containing the drug with the drug-free control. All isolates had low MICs (MIC90s £ 0.5 lg ml-1) for itraconazole and posaconazole, and most isolates were less susceptible (MIC90s ‡ 2 lg ml-1) to amphotericin B and fluconazole. The MEC range for all isolates was as follows: 0.25 to 8 lg ml-1 for caspofungin, and 0.008 to 4 lg ml-1 for micafungin. For C. guyanensis and P.europaea, for which species more than 10 isolates were included in the study, MEC50 and MEC90 values were calculated and found to be variable for these two species: C. guyanensis had MEC90 values of 2 lg ml-1 for caspofungin and 0.5 lg ml-1 for micafungin, while P. europaea had MEC90 values of 4 lg ml-1 for caspofungin and 0.25 lg ml-1 for micafungin. There were no significant differences in the activities of the surveyed drugs between and within the genera Cyphellophora and Phialophora.

abstract No: 

    • ISHAM 18th (2012)