In vitro activities of caspofungin and four other antifungal agents against Trichophyton spp. isolates

M. Demirbilek, F. Can, T. Gulec, Y. Saray, H. Arslan

Author address: 

Ankara, TR

Abstract: 

Background: In recent years the number of infections caused by dermatophytes has increased considerably. Pharmacotherapy for these infections still remains a problem as the necessity of long time appliance of antifungal agents. Caspofungin is a new antifungal agent that acts on the cell wall by inhibiting glucan synthesis. A standardized reference method for dermatophyte in vitro susceptibility testing and reproducible, clinically relevant method for caspofungin has not been fully established. The aim of this study was to evaluate the in vitro activity testing method of caspofungin and other antifungal drugs against clinical isolates of Trichophyton spp. from Ankara-Turkey. Methods: A total of 172 clinical isolates of Trichophyton spp. (136 T. rubrum, 29 T. mentagrophytes, and 7 T. tonsurans) were tested. The antifungal activities of caspofungin, fluconazole, itraconazole, terbinafine, amphotericin B were determined by broth microdilution tests performed mainly according to the NCCLS standards for filamentous fungi using RPMI 1640 as the test medium. Microscopic and macroscopic minimal effective concentration (MEC) values for caspofungin and MIC values for other drugs were determined after 5-7 day incubation. Results: Microscopic and macroscopic MEC values were mostly similar for caspofungin at all test conditions (100% agreement for T. tonsurans, 1.5% agreement for T. rubrum and 79% agreement for T. mentagrophytes). Caspofungin was more active against T. rubrum (MIC50, 0.06 μg/ml and MIC90, 0.5 μg/ml) than the others (MIC50, 0.25 μg/ml and MIC90, 1 μg/ml). Terbinafine was significantly more active (MIC50, 0.002 μg/ml and MIC90, 0.008 μg/ml) than the other drugs (p
2006

abstract No: 

P748

Full conference title: 

16th European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 16th (2006)