In Vitro Activities of Antifungal Agents Against Rhodotorula Species

T. Pelaez, B. Gama, P. Muñoz, M.J. Ruiz-Serrano, J. Guinea, R. Flores, M. Pedromingo, E. Bouza

Author address: 

Hospital Gregorio Marañón, MADRID, Spain

Abstract: 

Objective: Rhodotorula species has emerged as a pathogen that mainly infects immunosuppressed patients with or without a central venous catheter. Despite the increased incidence of invasive infection due to Rhodotorula species in the last decade, data on the antifungal susceptibility of this species are limited. This study reports the epidemiology and susceptibility data for the clinical isolates of Rhodotorula species in our institution. Methods: From 1988 to 2008, 252 isolates of Rhodotorula species were recovered from 250 patients. MICs were determined using the E-test. The antifungal agents tested were amphotericin B (AMB), fluconazole (FZ), itraconazole (IZ), voriconazole (VZ), posaconazole (POS), and caspofungin (CAS). Results: We divided the study into 2 periods: 1988-98 (86 isolates/86 patients) and 1999-2008 (166 isolates/164 patients). Most of the isolates (173, 69%) were identified as Rhodotorula mucilaginosa (formerly R. rubra), 59 (23%) as Rhodotorula glutinis, 5 (2%) as Rhodotorula minuta, and 15 (6%) as Rhodotorula species on the basis of morphological and biochemical features. Clinical isolates were obtained from a variety of clinical sources: skin (n=64), nails (n=63), tissue biopsy specimen (n=25), respiratory tract (n=24), oropharynx (n=24), abscesses (n=16), urine (n=10), CSF (n=8), peritoneal fluid (n=6), blood (n=5), vagina (n=5), and other sites (n=2). The MIC ranges (μg/ml) of all antifungal agents tested against the 41 available clinical isolates of Rhodotorula species from deep sites were as follows: AMB (0.5-2), FZ (8- >256), IZ (0.06-16), VZ (0.03-8), POS (0.01-16), and CAS (4->32). The geometric means (GMs) of the MICs and MICs90 were: AMB (1.6/2), FZ (44.1/>256), IZ (0.6/16), VZ (0.5/8), POS (0.3/16), and CAS (12.2/>32). Most of the isolates were inhibited by
2009

abstract No: 

P020

Full conference title: 

4th Trends in Medical Mycology
    • TIMM 4th (2012)