The velvet-like complex from chrysogenum: A regulatory network of five subunits controls secondary metabolism and morphogenesis.

Kück U1, Hoff B1, Kamerewerd J1, Kopke K1, Wolfers S1, Katschorowski A1, Milbredt S1, Koutsantas K1, Kluge J1, Zadra I2, Kürnsteiner H2

Author address: 

Christian Doppler Laboratory for Fungal Biotechnology, Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr-Universität Bochum, Universitätsstraße 150, 44780 Bochum; 2Sandoz GmbH, 6250 Kundl, Austria

Abstract: 

The global regulator velvet together with laeA is a core component of the velvet complex from Aspergillus nidulans. We have characterized a velvet-like complex from the penicillin producer Penicillium chrysogenum, which contains at least five different subunits. Included is PcvelA P. chrysogenum, which is an inhibitor of light-dependent conidiation and affects the biosynthesis of the beta-lactam antibiotic penicillin*. We will present an extensive analysis of subunits PcvelB, PcvelC and PcvosA of the velvet-like complex, including data from array hybridization, high performance liquid chromatography, quantification of penicillin titers, microscopic investigations and mass spectrometry. We provide evidence that all subunits of this complex have conserved as well as novel roles in secondary metabolism and morphogenesis in P. chrysogenum. These results confirm and extend the current picture of regular networks controlling both, fungal secondary metabolism and morphogenesis. *Hoff et al. EUKARYOTIC CELL 9: 12361250 (2010)
2011

abstract No: 

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Full conference title: 

26th Fungal Genetics Conference
    • Fungal Genetics Conference 26th (2005)