Variation of galactomannan optical density index for Aspergillus species in samples of bronchoalveolar lavage fluid

Anna Malchikova

Abstract: 

Background: To estimate of  galactomannan antigen level in samples of bronchoalveolar lavage fluid (BAL) colonized with various Aspergillus spp.

Material/methods: The retrospective study was carried out from January 2010 to November 2015.   The BAL samples tested for galactomannan antigen level (GM, Platelia Aspergillus, Bio-Rad, USA) with the incidence ofAspergillus spp were analysed.

Results: A total of 337 BAL samples was evaluated. Among 202 (60%) strains of isolated Aspergillusspp there were 183 (90,6%) strains obtained by monoculture and 19 (9,4%) obtained by mix culture. Distribution of Aspergillus species in monoculture was following: A. fumigatusn=54(30%), A. flavusn=38(21%), A. niger n=28(15%), A. sydowii n=16(9%), A. versicolorn=13(7%), A. niveusn=6(3%), A. nidullans n=6(3%),A. ochraceus n=5(2,7%), A. candidus n=4(2%),  A. alliaceusn=3(1,3%) ,  and rare  Aspergillus species (A. carneus, A. oryzae, A. restrictus) n=8(6%). The positive values of opticaldensity index for GM (cutoff˃1) were identified for 68% A. flavus, 48% A. fumigatus, 31%A. sydowii, and 21% A. niger. The results of the retrospective study were summarized in table.  The statistical estimation for the positive GM in samples was following:  p=0.05 and p=0.005 for prevailingincidence of A.flavus than A. fumigatusandother Aspergillus spp., respectively, and p=0,00001  forA. fumigatus than the others. The values of optical density index for GM were negative (cutoff ˂1) in 41% of total samples with prevailing among the other Aspergillusspp. (75%).

Conclusions: The variation of galactomannan optical density index was reported for different Aspergillus species in samples of bronchoalveolar lavage fluid. Among the isolates the strong correlation between the positive values for GM and the incidence of Aspergillusspp.  was detected for A. flavus, A. niger, A. versicolor(р<0,05). The occurrence of the positive values for GM and the incidence of Aspergillus species was the highest for A. flavus (68%). 

Tables: 

2016

abstract No: 

#3387

Full conference title: 

European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 26th (2016)