Value of antigen detection in diagnosis and follow-up of invasive aspergillosis

A.Sulahian, F.Boutboul, P.Ribaud, T.Leblanc, C.Lacroix, F.Derouin


Objectives: To evaluate over a 4 year period the value of an ELISA technique for the research of galactomannan (GM) for the diagnosis and follow-up of invasive aspergillosis (IA). Methods: The study evaluated the specificity and sensitivity of the detection of GM in 6209 sera from 797 hematology patients. Serial screening of GM was performed using a double sandwich ELISA assay (Platelia Aspergillus Sanofi Diagnostics Pasteur). Fifty-three patients presented confirmed or probable IA. Results: Antigen was detected on at least two sequential sera in 48/53 patients, giving a sensitivity of 90.6%. GM antigenemia was detected before the onset of radiological signs in 31/48 (64.6%) of patients with a mean of -8.4 days, and before clinical symptoms in 18/48 (39.6%) patients with a mean of -6.9 days. In patients without IA, 44/744 had positive antigenemia resulting in a specificity of 94%. False positive results could not be related to the presence of concurrent mucositis.

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11th European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 11th (2001)