The Use Of PCR In The Diagnosis Of Aspergillus Disease In Lung Transplant Recipients.

N.R. Simler, S. Lok, A. Fox, B. Oppenheim, M. Jones, N. Yonan, J.J. Egan


The diagnosis of invasive pulmonary aspergillosis (IPA) is difficult, and culture positive results often represent colonisation. We evaluated aspergillus (ASP) mitochondrial DNA PCR, combining a solution hybridisation assay in a microtitre plate format with colorimetric end stage detection (PCR-ELISA) for the identification of IPA. Bronchoalveolar lavage (BAL) specimens and peripheral blood EDTA were studied. BAL and EDTA samples were sent for PCR analysis in addition to standard culture and cytological examination for ASP. There were 48 patient episodes for BAL and 45 episodes for EDTA. The results are shown in the table below:The positive predictive value for IPA was 12.5% and 0% for BAL and EDTA PCR respectively. The negative predictive value for IPA was 97.5% and 97.2% for BAL and EDTA PCR respectively. This study suggests that BAL and EDTA PCR may be of limited value for the detection of IPA in lung transplant recipients.

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The American Lung Association - American Thoracic Association Conference,1998
    • ALA-ATA