Transposon Vader: From a mobile element towards a molecular tool

Frank Kempken, ElkBir Hihla

Author address: 

Botanisches Institut, Christian-Albrechts-Universität, Olshausenstraße 40, 24098 Kiel, Germany

Abstract: 

Transposons are mobile DNA sequences, which are found in all eukaryotic genomes (1-3). We have analyzed the transposon content in two fungal genomes, Aspergillus niger and Penicillium chryosogenum. One non-autonomous element, the A. niger transposon Vader, was shown to be active during strain development (4). Vader mobility could also be shown in a transposon trap experiment. Due to its obvious activity and to its ability to insert into genes Vader appears to be suitable as a gene tagging tool. A vector was constructed which carries a Vader element between the promoter and open reading frame of the hygromycin resistance gene. The Vader element was modified by adding a unique oligonucleotide binding sequence. Conidiospores were plated on hygromycin B containing media which allows for selection of excision of transposon Vader. Reintegration sites were determined using TAIL-PCR. Vader transposition shows a high insertion rate into genes which appears very promising. At current we analyse a large number of integration sites to determine any target site preferences. In addition we will introduce Vader into A. nidulans and other heterologous hosts. Kempken F, Kück U (1998) BioEssays 20:652-659 Kempken F (2003) In: Arora DK, Khachatourians GG (eds) Applied Mycology and Biotechnology, Vol. 3 Fungal Genomics, Elsevier Science Annual Review Series, pp83-99 Pöggeler S, Kempken F (2004) In: Kück U (ed) The Mycota II, Genetics and Biotechnology, 2nd edition, Springer Verlag, Heidelberg, New York, Tokyo, pp165-198 Braumann I, van den Berg M, Kempken F (2007) Fungal Genet Biol 44:1399-1414
2010

abstract No: 

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Full conference title: 

10th EUROPEAN CONFERENCE ON FUNGAL GENETICS
    • ECFG 10th (2010)