Th,/Th2 T Cells and Cytokine Synthesis in Cystic Fibrosis Patients with Allergic Bronchopulmonary Aspergillosis

A. P. Knutsen ], P. Hutchinson ], J. Smick I, V. P. Kurup2

Author address: 

IAllergy and Immunology, St. Louis University, St. Louis, MO, 2Allergy and Immunology, Medical College of Wisconsin, Milwaukee, Wl.

Abstract: 

RATIONALE: ABPA is characterized by a heightened Th2 CD4+ T-cell response to A. fumigatus allergens compared to CF patients without ABPA. We hypothesize that one reason for this response is skewed Th2 Tcell responses in patients with ABPA. METHODS: PBL were stimulated non-specifically with PMA/IO and for antigen-specific responses with tetanus toxoid and Asp f2/f3/f4. PBL were examined for cytoplasmic cytokines and tbr culture supernatant cytokine synthesis. RESULTS: The percentages of IFN-g+ CD3+T cells following PMA/IO stimulation were significantly decreased in both ABPA and non-ABPA patients compared to controls. There were no significant differences of 1L- 4+ CD3+ T cells between ABPA and non-ABPA patients. When tetanus toxoid and Aspergillus stimulated T cells were examined, both ABPA and non-ABPA patients had significantly decreased IFN-g+ CD3+ T cells compared to controls. ABPA patients also had significantly decreased IFN-g culture supernatant synthesis in both tetanus toxoid and Aspergillus stimulated cultures. In Aspergillus stimulated T cells, ABPA patients had significantly increased IL-4+ CD3+ T cells compared to non-ABPA patients and controls. IL-5 synthesis was significantly increased in ABPA CF patients in Aspergillus stimulated cultures. IL-10 synthesis was markedly increased in Aspergillus but not tetanus toxoid stimulated cultures in ABPA and non-ABPA CF patients and normal controls. CONCLUSIONS: ABPA CF patients had increased percentages of Th2 T-cells and increased IL-5 synthesis in Asp f2/f3/f4 stimulated cultures. In addition, Aspergillus allergens induced IL-10 synthesis in both ABPA and non-ABPA CF patients, skewing a Th2 response to A. fumigatus colonization in ABPA patients. Funding: Cystic Fibrosis Foundation
2003

abstract No: 

S312

Full conference title: 

2003 American Academy of Allergy, Asthma, and Immunology Annual Meeting
    • AAAAI 2003 (59th)