Th2-Type Innate Immune Response In The Airways Induced By Aspergillus Extracts

K. Tomomatsu , T. Oguma , S. Kagawa , K. Fukunaga , Y. Suzuki , M. kodama , H. Ogura , N. Kamiishi , J. Miyata , K. Tanaka , H. Kabata , K. Sayama , K. Asano

Author address: 

Keio University School of Medicine, Tokyo, Japan, Keio University School of Medicine, Shinjyukuku Tokyo, Japan


[Rationale] Recent studies have shown that Th2-type innate immune response can be induced in the gut by parasite infection, or cytokines such as interleukin (IL)-25 or IL-33. We found a similar Th2-type innate immune response in the airways exposed to fungal allergen. In the present study, we studied the immunological mechanism of this Th2-type response. [Methods] C57BL/6 mice (WT) and Rag2 deficient mice (Rag2KO) were daily exposed to extracts of Aspergillus fumigatus (Af) or IL-33 for 3 days. On days 1, 4, 7 after the final exposure, bronchoalveolar lavage (BAL) was performed and the lungs were harvested. In some mice, mice were injected with soluble ST2Fc-fusion proteins to inhibit IL-33 function, or with anti NK1.1 antibody to deplete NK/NKT cells. [Results] In WT mice, Af extracts induced eosinophilic airway inflammation accompanied with a sustained IL-5/IL-13 release and increased mucus production. This response was diminished in Rag2KO mice, but was not affected by the treatment with soluble ST2Fc-fusion proteins or anti-NK1.1mAb. On the other hand, Th2-type inflammation induced by IL-33 was unchanged between WT mice and Rag2KO mice. [Conclusion] Th2-type innate immune response induced by Af extracts was IL-33 independent, but dependent on T/B lymphocytes.

abstract No: 


Full conference title: 

American Thoracic Society
    • ATS 2011