Synergy Studies of the Fungicial Plant Saponin, CAY-1, with Silver and EDTA.

A. J. DE LUCCA II, T. E. CLEVELAND, M. P. LOVISA;

Author address: 

Southern Regional Res. Ctr., USDA, ARS, New Orleans, LA.

Abstract: 

Background: CAY-1 is a fungicidal saponin produced by Capsicum species. Silver (Ag) and EDTA inhibit Aspergillus and Fusarium growth. This study determined Ag and EDTA fungicidal properties for several Aspergillus and Fusarium species both alone and with CAY-1. Methods: CAY-1 was purified by column chromatography and HPLC/MS. Silver nitrate and EDTA was dissolved in 1% potato dextrose agar (PDB). Nongerminated (NG) and germinating (G) conidia (25μl) of A. flavus (AFLAV), A. fumigatus (AFUM), A. niger (AN), F. moniliforme (FM), F. oxysporum (FO) and F. solani (FS) were incubated separately (30 min, 30°C) with CAY-1 (0-31μg/ml), Ag (0-175μg/ml), and EDTA (0-2400μg/ml). Final volume was 250μl. Final conidial concentration was 1.2 X 104/ml. Post incubation, 50μl aliquots were spread on PDA plates, incubated (48 hr, 30°C) and viable colonies counted. Controls consisted of broth (225μl) and NG or G conidia (25μl).Synergy studies consisted of CAY-1of at least the second non-lethal concentration below the minimum lethal dose mixed with sub-lethal Ag or EDTA doses. Synergy controls (250μl) consisted of NG or G conidia (25 μl) in PDB only or CAY-1 at the test level. Remaining protocol was as described above. SigmaStat was used to determine statistical significance (p
2007

abstract No: 

F1-1672

Full conference title: 

47th Interscience Conference on Antimicrobial agents and Chemotherapy
    • ICAAC 47th