Activation of the fungal cell wall integrity pathway is a mechanism used by fungi to escape from cell wall threatening conditions. In Aspergillus niger we have previously shown that the agsA gene, encoding an í¡-1,3-glucan synthase submit is transcriptionally activated in response to cell wall stress, and that this response is mediated via a RLM A dependent signal transduction pathway. W e have used the agsA promoter region to set up a GFP-based reporter system to identify compounds that activate the agsA expression by fusing the PagsA to the cytosolic GFP or to a nuclear targeted version of GFP. The reporter strains confirmed earlier observation that the agsA gene is activated in response to various cell wall disturbing compounds such as CFW , caspofungin and tunicamycin. Other forms of stress (osmotic or oxidative stress) did not activate agsA expression, indicating that the induction of agsA is cell wall stress specific and not a general stress response. W e have used the cell wall stress reporter system to study the effect of 20 known antifungal compounds in relation to cell wall remodeling.
Full conference title:
23rd Fungal Genetics Conference
- Fungal Genetics Conference 23rd (2002)