Species Distribution and Antifungal Susceptibility Profile of Candida Species and Cryptococcus neoformans Isolates: Two Years of Croatian Surveillance

E. Mlinaric-Missoni, V. Vazic-Babic, E. Missoni

Author address: 

Croatian Institute of Public Health, ZAGREB, Croatia


Objectives: To conduct a prospective surveillance of species distribution and in vitro susceptibility to 5-fluorocitosine (5-FC), amphotericin B (AMB), fluconazole (FLU), itraconazole (ITR), and voriconazole (VOR) among Candida species and Cryptococcus neoformans isolates associated with deep-seated and disseminated infections during a 24 month-period in patients from seven Croatian medical institutions. Methods: From January 2007 until December 2008 we were able to collect a total of 685 yeast isolates: 650 (95%) isolates of 13 Candida species and 35 (5%) isolates of C. neoformans from five tertiary care hospitals, one general hospital and the Croatian National Institute of Public Health (CNIPH). The isolates were collected at the individual study sites and were sent to the National Reference Laboratory for systemic mycoses (in CNIPH) for identification and susceptibility testing. Yeast isolates were identified by ID 32 C (BioMerieux) and classical methods. Antifungal susceptibility profile of isolates was determined by ATB FUNGUS 3 (BioMerieux) microdilution methods following the manufacturer's instructions. MIC readings were performed visually and the results were interpreted according to the CLSI recommendations. Results: The collection included 115 (16.8%) bloodstream or CSF isolates of Candida species and C. neoformans and 570 (83.2%) isolates of Candida species from other clinical samples (BAL, sputum, urine, aspirates, drainage swabs, stools). Candida species distribution was as follows: C. albicans 360 (55%), C. glabrata 110 (17%), C. parapsilosis 63 (10%), C. krusei 35 (5%), C. tropicalis 25 (4%) and other Candida species 57 (9%). Only 0.3% C. albicans and 8.6% C. krusei isolates were resistant to 5-FC. All Candida species and C. neoformans isolates were sensitive to AMB. Isolates of C. albicans, C. tropicalis, and C. neoformans

abstract No: 


Full conference title: 

4th Trends in Medical Mycology
    • TIMM 4th (2012)