Abstract:
Background: Human candidiasis is increasing now in incidence and in clinical manifestation, due to the
high frequency of prolonged antibiotic therapy, the invasive procedures and immune deficiencies. The
immunosuppression by transplant, the drug therapy for cancer and AIDS favour the disseminated
infections. Knowledge of clinical manifestations and predisposing factors is profitable in order to establish
the proper prophylaxis and treatment.
Objectives: Patients hospitalized for clinical assumption of candidiasis were assessed. The aim of this
study was to evaluate species distribution and antifungal susceptibility of Candida isolates recovered from
pathological products, at some hospitals in Cluj-Napoca.
Methods: In a period of 2 years, 338 Candida isolates were collected. All isolates of Candida spp. were
identified by the germ tube test and the API test (bioMerieux). Identification of species was followed by
examination with broth the microdilution method, as described in NCCLS M27-A2, of the antifungal
susceptibility to six agents, with readings after 24 and 48 h of incubation. MIC of antifungal agents was
determined by the E test.
Results: The candidiase aetiology was established in 338 patients. 70% of the candidiasis were
nosocomial infections, 5% occurred in patients hospitalized in an intensive care unit, 25% in patients
hospitalized for cancer therapy. Mucosal candidiasis prevails (63.1%) followed by cutaneous candidiasis
(25.2%) and disseminated candidiasis (10.8%). The overall species distribution was:41%C.albicans,
22.3%C. parapsilosis, 12.4%Candida glabrata, 12%Candida tropicalis, 2% C. krusei, 0.7%C.
guilliermondii, and 5.8%Candida spp.
Conclusions: An important issue in candidiasis therapy is the occurrence of the resistant strains, both to
common antifungal drugs and to those recently discovered. Our data suggest that the Candida species
distribution and the antifungals resistance rate are similar to those reported previously in USA and Europe,
but disparities in the species distribution and in the antifungal susceptibility of Candida isolates from other
studies and countries are described. Our future goal is to study the susceptibility of Candida to antifungal
drugs in order to struggle against these wide spread infections. The findings emphasize the need for
continuous surveillance and further clinical investigational studies.
2006
abstract No:
R2165
Full conference title:
16th European Congress of Clinical Microbiology and Infectious Diseases
NULL
- ECCMID 16th (2006)