Background: Candida species are considered a major cause of nosocomial bloodstream infections worldwide with an increase in isolation of non-albicans Candida species. The purposee of the study was to evaluate the species distribution and antifungal susceptibility profiles of Candida species recovered from blood in a tertiary teaching hospital in Saudi Arabia during a 5-year period (2010- 2014).
Material/methods: Candida species recovered from blood cultures during a 5-year period (2010- 2014) were included. All isolates were identified to species level by the germ tube test and the API 20 C AUX (bioMerieux). Antifungal susceptibility of Candida isolates to amphotericin B, fluconazole, voriconazole, caspofungin, and 5-flucytosine was evaluatedd using Sensititre YeastOne and the MIC breakpoints for resistance were based on CLSI.
Results: A total of 167 isolates of Candida species were recovered during the study period. Candida albicans was the predominant species (30.5 %), followed by Candida glabrata (24.5 %), Candida parapsilosis (19.8 %), Candida tropicalis (19.1 %), Candida krusei (2.4 %) and other species (1.8%). All isolates were susceptible to amphotericin B and only two isolates, one C. tropicalis and one C. glabrata exhibited an MIC for amphotericin B of >1 mg/L. Resistance to fluconazole was observed in 5.9 % of C. albicans, 5% in C. glabrata and 15.2 % of C. parapsilosis. Most of the isolates were susceptable to voriconazole. Resistance was detected in C. albicans (3.9%), C. glabrata (2.5%) and C. parapsilosis (3%). For C. trpoicals (9.4%) and C. parapsilosis (9.1%) were susceptible dose dependent (SDD). Resistance to caspofungin was observed in C. glabrata (5%), C. krusei (25%) and C. albicans (1.96%). 15% of C. glabrata and 6% of C. parapsilosis were intermediate.
Conclusions: High rate of Non-C. albicans species was observed which constituted 69.5 % of the isolates. Although the rates of resistance were low, a considerable number of isolates were classified as SDD or intermediate for fluconazole and caspofungin respectively. Therefore, continuously surveillance of species distribution and antifungal susceptibility patterns of yeasts is needed.
Full conference title:
- ECCMID 26th (2016)