SEROTYPES DISTRIBUTION AND DRUG SUSCEPTIBILITIES OF CRYPTOCOCCUS NEOFORMANS AND CANDIDA ALBICANS FROM CLINICAL SOURCES IN NAIROBI, KENYA

Bii CC, Revathi G

Author address: 

Kenya Medical Research Institute, Nairobi, Kenya

Abstract: 

The HIV epidemic in Africa has led to an increase in the incidence and morbidity due to opportunistic fungi. Cryptococcal meningitis and Candida albicans infections are some of the most commonly encountered conditions in clinical practice in Kenya. It is estimated that up to 40% of HIV/AIDS develop cryptococcal meningitis. Due to the high frequency and the severe clinical manifestations of Cryptococcus infection, we determined the serotype distributions and drug susceptibility profiles of Cryptococcus neoformans and Candida albicans from clinical sources. Twenty-eight C. neoformans isolates from cerebrospinal fluids of patient with meningitis and thirty-two Candida albicans from sputum, blood culture, broncho alveolar lavage fluid and urine were serotyped and drug susceptibility determined. Preliminary identification was done on ChromAgar Candida and confirmed by Api 20 C aux (bioM’erieux, France). Serotyping was done by slide agglutination test using Candida and Crypto Check antisera (Iatron Co., Japan). Candida susceptibility to amphotericin b, fluconazole, 5-fluorocytosin, and miconazole was done using Asty antifungal susceptibility testing for yeasts (Asty, Kyokuto, Japan). Susceptibility of Cryptococcus to amphotericin B was determined using broth microdilution technique. All C. albicans isolates were serotype A while 19/32 (59.4%) had MIC ≥ 1μg/ml to amphotericin b. Only one strain of C. albicans had MIC 8804; 64μg/ml to 5-fluorocytosin while 2 and 6 strains were susceptible dose dependent (MIC of 16-32 μg/ml) to fluconazole and miconazole respectively. Most [9/28 (32.1%)] C. neoformans isolates were serotype A the rest were either serotype D, AD or AB but there were 9/28 isolates which did not agglutinate with the typing sera. Seventy five percent of the C. neoformans isolates had MIC of 0.5 μg/ml to amphotericin b while 4 isolates had MIC of ≥ 1 μg/ml. The rest of the isolates had MIC of
2003

abstract No: 

none

Full conference title: 

13th Annual Focus on Fungal Infections
    • FFI 13th (2003)