Semi-national surveillance of fungaemia in Denmark: species distribution and anti-fungal susceptibility

M.C. Arendrup, K. Fuursted, B. Gahrn-Hansen, I.M. Jensen,J.D. Knudsen, B. Lundgren, M. Tvede, H.C. Schønheyder

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Objective: The epidemiology of fungaemia in Denmark is not systematically investigated. This study was undertaken to characterize species distribution and susceptibility pattern in different parts of the country. Methods: Six departments of clinical microbiology in Copenhagen, Copenhagen county, Funen county, Aarhus county and Northern-Jutland county were included prospectively. The 6 departments serve a population of 2,870,000which is about half the Danish population. From each episode of fungaemia, defined as isolation of the same fungus from blood culture with in a 21 day period, one isolate was to be sent to the unit of mycology and parasitology for verification of species identification and susceptibility testing. Species identification was done by use of colony morphology on CHROMagar plates at 35C (CHROMagar CO.,Paris, France), presenceor absenceof chlamydoconidiaonRice and Tween agar plates (SSI Diagnostica, Hilleroed, Denmark), growth at 35 and 42C and by ID32C (bioMerieux, Marcy l’Etoile, France). Susceptibility testing for amphotericin B, caspofungin, fluconazole and itraconazole was performed according to the NCCLS document M27-A. Results: A total of 298 episodes of fungaemia were registered. Candida species accounted for 97.5% of the fungal pathogens. Although C. albicans was the predominant species (64%) the proportion was lower than previously described (85% in 19945). However, the relative proportion varied considerably, i.e. from

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    • ECCMID 15th (2005)