The screening of some medicinal plants for antifungal activity by Bioautography agar method

B. Sadeghi-Nejad1, S.S Deokule2

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Background: The bioautography agar overlay method is an improved version of a disc diffusion method. It is considered as one of the most efficient methods for the detection of antimicrobial compounds. In this study the bioautography agar overlay method was performed for the detection of antifungal compounds of the ethyl acetate extracts of Hyptis suaveolens leaf, Pogostemon parviflorus leaf, Ixora brachiata root, Ixora brachiata leaf, Drynaria quercifolia Rhizome and Satureja khuzestanica leaf against Trichophyton rubrum and Microsporum gypseum that those were showed in-vitro antidermatophytic activity. Material and Methods: In bioauthography method, the ethyl acetate extracts were prepared by maceration and evaporating to dryness. Then Rf values of separated compounds were determined with systems viz.ethyl acetate/methanol/water (40:5.4:5): [EMW] (polar/neutral); chloroform/ethyl acetate/for-mic acid(5:4:1): [CEF] (intermediate polarity/acidic); benzene/ethanol/ammonium hydroxide (90:10:1): [BEA] (non-polar/basic) and acetone/ethyl acetate/petroleum ether (0.5: 0.5:2.0) [AEP]. Results: In all tested plants Rf values of a active antifungal compound were 0.90-0.98 in acetone/ethyl acetate/petroleum ether (0.5:0.5:2.0) against Trichophyton rubrum and Microsporum gypseum. Among of four separation systems were used, only the solvent system of AEP gave the best resolution of spots in TLC analysis because antifungal compounds were relatively semi-polar and therefore did not separate well in the polar eluent EMW. The ethyl acetate extracts of H. suaveolens, P. parviflorus and Ixora brachiata leaf seprated with AEP possess few compounds with high activity against M. gypseum and T. rubrum at Rf values of the active compounds of 0.57-0.98. The clear spots indicate present of antifungal compounds that it inhibited the growth of tested fungi. Conclusion: A bioautography is an important detection method for a new or unidentified compound, because it is based on the biological effects of the substances under study. Keywords: Medicinal plants, antifungal activity, bioautography, Rf value

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Full conference title: 

Iranian Congress on Medical Mycology
    • ICMM 2011