Screening of Lake Actinomycetes for the Production of Antifungal and Antialgal Compounds

S. Aslam, I. Sajid

Author address: 

Univ. of the Punjab, Lahore, Pakistan, Lahore, Pakistan

Abstract: 

Background: Although chemical synthesis and engineered synthetic compounds are accelerated in the production process, nature still has an edge for its versatile pharmaceuticals especially from the wetland sediments. The lake sediments for isolating actinobacteria have not been extensively investigated although its ubiquitous presence and diversity remains inexhaustible than its terrestrial counterparts. The present study was designed to screen the lake actinomycetes for the production of antifungal and antialgal compounds.Methods: A total of forty strains of actinomycetes were isolated from the water and sediment samples of a saline Kallar Kahar lake of Pakistan. The identified strains were cultivated on small scale as shaking cultures and crude extracts were obtained by ethyl acetate extraction. These crude extracts were screened against Candida albicans, Candida tropicalis, Chlorella vulgaris by agar well diffusion method. In chemical screening the crude extracts were analyzed by TLC and HPLC-UV analysis.Results: The results revealed that majority of the isolated strains (90%) belong to the genus Streptomyces and some of the strains were of Micromonospora. Many lake actinomycete isolates showing remarkable antifungal and antialgal activity. However the strains KL36, KL117, KL37, KL103, KL51, KL71, KL83 and KL91 exhibited appreciable zones of inhibition of 15mm, 16mm, 17mm, 18mm, 21mm, 23mm, 23mm, 23mm respectively against Candida tropicalis while the isolates KL23, KL14, KL212, KL71, KL91, KL112, KL117, KL51, KL29, KL103 and KL36 exhibited maximum activity against Candida albicans. Approximately 80% of the isolated strains were observed to exhibit the potential antialgal activity against Chlorella vulgaris. Among the isolated strains KL36, KL37, KL41, KL59, KL52, KL116 and KL98 showed maximum zone of inhibition of 16mm, 18mm, 17mm, 19mm, 21mm, 17mm, 15mm respectively. On the other hand, KL84, KL94, KL43, KL51 and KL38 gave no zone of inhibition against Chlorella vulgaris. In thin layer chromatographic analysis and HPLC-UV analysis, crude extracts of the isolates also showed an impressive chemical diversity of lake actinomycetes.Conclusions: Lake actinomycetes are a potential source of antifungal and antialgal compounds which can be exploited for further purification of unique secondary metabolites in drug discovery.
2016

abstract No: 

SUNDAY-428

Full conference title: 

ASM Microbe 2016
    • ASM microbe 1st (2016)