Salt stress mediated regulation of glucose 6 phosphate dehydrogenase and glyceraldehyde 3 phosphate dehydrogenase in halotolerant Aspergillus repens.

Phullara Shelat, Surobhi Lahri, Rajiv Vaidya and H.S. Chhatpar


Aspergillus repens which was isolated earlier from salt pans was found to show significant growth under salinity conditions (2M). Significant increase was observed in the activities of glyceraldehyde 3 phosphate dehydrogenase and glucose 6 phosphate dehydrogenase under salt stress conditions as compared to control. Osmolytes betaine and proline did not have any influence on G6PD activity from control whereas under salt stress conditions, both these osmolytes caused reduction in enzyme activities. Glycine and glycerol however were found to decrease the activity in both control and salt stress conditions. Asp. repens was earlier found to accumulate Na+ and K+ when grown under salt stress conditions. In vitro conditions sodium and potassium were found to increase the activity of glyceraldehyde 3 Phosphate dehydrogenase, high concentrations were however found to be inhibitory. Potassium, magnesium and calcium were found to be inhibitory to glucose 6 P dehydrogenase activity from both control and salt stress conditions. Fatty acids like oleic and myristic were found to be inhibitory to glucose 6 phosphate dehydrogenase activity. Two dimensional electrophoresis showed induction and repression of some proteins under salt stress condition.

abstract No: 

Fungal Genet. Newsl. 50 (Supl):abstract

Full conference title: 

22nd Fungal Genetics Conference
    • Fungal Genetics Conference 22nd (2001)