Purpose In patients with allergic bronchopulmonary Aspergillosis (ABPA), there is a robust eosinophilic inflammation systemically and locally in the airways. We hypothesized that Aspergillus species, in addition to serve as an allergen, activate various innate immune reactions with the pathogen-associated molecular pattern(s) and augment allergic airway inflammation. In the present study, we evaluated the role of fungal extracts on the ovalbumin (OVA)-induced allergic airway inflammation. Methods C57BL/6 mice (WT) and IL-17 deficient mice (IL-17KO) were sensitized and exposed to OVA. During the exposure phase, extracts of Aspergillus fumigatus (Af) was administered intranasally with OVA. On day 1, 4, 7 after final administration, bronchoalveolar lavage (BAL) was performed and lungs were harvested. Results In OVA-exposed WT mice, co-administration of Af extracts synergistically enhanced eosinophilic airway inflammation accompanied with a sustained IL-13 release and increased mucus production on day 7. In IL-17KO mice, the effects of Af extracts appeared earlier (on day 4) than in WT mice. Conclusion Af extracts positively and negatively modulates allergic airway inflammation through the activity of IL-13 and IL-17.
Full conference title:
20th European Respiratory Society conference
- ERS 20th (2010)