Role of Candida in immunomodulations in patients suffering from pemphigus

Shukla Das*. SA Dar,VG Ramachandran, N Bhattacharya, B Banerjee,V Sood and A Banerjea

Author address: 

Department of Microbioloy, UCMS & GTB Hospital, Dilshad Garden, Delhi, 110 095, India

Abstract: 

Pemphigus, an autoimmune skin disease characterized by intraepithelial blisters, induced by autoantibodies directed against desmosomal adhesion proteins. B cells and T cells play a major role in regulation and induction of specific immune response against infectious agents. Role of superantigens and Candida recall antigens in provoking polyclonal activation of T cells including autoreactive T cells is still unknown. The study was initiated to investigate response of T cells (CD4+/CD8+) to Candida antigens and the role of memory cells CD45RO & CD45RA in modulating the autoantibody response in pemphigus patients as compared to healthy individuals. 10 ml blood was collected from 25 adult patients of pemphigus and 10 healthy individuals. PBMCs were separated and 1 x 106 cells were incubated with 1-10 ug/ml PHAM, 10-100 ng/ml C. albicans in a 12 well cell culture plate with RPMI1640 at 37°C & 5% C02 x 72 hrs. Cells were washed and stained with appropriate monoclonal antibodies & scanned with BD FACS Calibur. Patients of pemphigus had decreased level of naive CD45RA + RO- cells as compared to healthy. CD3+/CD4+ T cells were elevated and isoform switch over from CD45RA to CD45RO indicates a rise in memory cells due to constant Candida antigen trigger from the skin. ’Corresponding author E-mail:[email protected]
2014

abstract No: 

OP-010

Full conference title: 

Society for Indian Human and Animal Mycologists 2014
    • SIHAM