Red blood cell antioxidant levels after treatment with diethyl carbamazine citrate in persons with asymptomatic microfilaraemia

R. Premaratna, T. G. A. N. Chandrasena, W. Abeyewickreme, N. R. de Silva, L. G. Chandrasena, H. J. de Silva


Background: The microfilaricidal action of DEC is poorly understood. In vitro studies have shown that, DEC stimulates platelets to release unspecified free radicals, which have microfilaricidal effects. In amicrofilaraemics living in endemic areas, red cell glutathione peroxidase (GPX) levels are significantly higher than in asymptomatic microfilaraemics. Catalase and superoxide dismutase (SOD) levels between these two populations are not different. This suggests a possible role for GPX related free radical species in the clearance of microfilaraemia. Aim: To study, in vivo, the changes in red cell GPX, catalase and SOD in asymptomatic microfilaraemic patients after treatment with DEC. Methods: Ten patients [(6 males), mean age: 29.8 years (range 16-57)] with asymptomatic microfilaraemia were tested for red cell GPX, catalase and SOD levels using spectrophotometry before and on the 3rd, 7th and 14th day during a 14-day course of DEC. 2 mL of venous blood was collected into an EDTA bottle between 8.00 and 9.00 am on each day. Assays were done within 6 h ofcollection.Blood wasstoredat8'baCuntilanalysis.

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11th European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 11th (2001)