Rapid identification of Candida species by MALDI-TOF technology

B. Nonnemann*, S.H. Hartzen, M. Tvede, T. Bjarnsholt

Author address: 

(Esbjerg, Copenhagen, DK)

Abstract: 

Objectives and background: Rapid identification of yeast isolates is important in order to adjust the antifungal treatment and to reduce patient morbidity and mortality. In this study we investigate whether Matrix assisted laser desorption/ ionization time of flight mass spectrometry (MALDI-TOF MS) can (i) replace current routine methods in first line identification of Candida, (ii) examine differences in generated results by two mass spectrometry instruments situated in two routine laboratory settings, (iii) measure time from entry of sample in the instrument to results. In East Denmark, the Microflex LT (Bruker Daltonics, Germany) and in West Denmark, the Vitek MS (bioMe’rieux, France). Method: Retrospective analysis of Candida isolates with identification in parallel by two MALDI-TOF instruments and API. API 20 C AUX (bioMe’rieux, France) was used as the standard method. Results: Ninety-three isolates were examined on Microflex LT with the Biotyper 2.0 software according to the manufacturer¢s manual.
2012

abstract No: 

NULL

Full conference title: 

22nd European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 22nd (2012)