With the rising frequency of fungal infections, as well as increasing reports of resistance to antifungal agents, major difficulty in treating fungal diseases is the availability of limited antifungal compounds. Antifungal activity has been detected in many soil bacteria, both saprophytes and human pathogens especially those belonging to the genera Bacillus and Pseudomonas. A variety of methods have been used to detect this activity in vitro. Presumably, this activity confers an ecological advantage on a bacterial population which competes with other species in a particular habitat. Multi-dimensional approach to chromatography is an important new addition to the analytical techniques. By combining the separation power of HPLC and the ability of MS to give useful structural information, it is possible to obtain preliminary data about the composition of a bacterial metabolite before any costly and time-consuming isolation work is performed. LC-MS provides a rapid method for avoiding known fungicidal substances since these can be screened out at the beginning of any investigation. When post-column derivatization is added, a good idea of the substitution pattern can be obtained. This review highlights the status of antifungal metabolites originated from bacteria with emphasis on their isolation, purification and identification.
Full conference title:
Iranian Congress on Medical Mycology
- ICMM 2011