PULMONARY DEPOSITION AND PROPHYLACTIC EFFICACY OF NEBULIZED FUNGIZONE AND AMBISOME IN INVASIVE ASPERGILLOSIS IN LEUKOPENIC RATS

E.J. Ruijgrok, E.W.M. Etten, I.A.J.M. Bakker-Woudenberg, A.G. Vulto

Abstract: 

We determined the pulmonary deposition and prophylactic efficacy of nebulized amphotericin B desoxycholate (Fungizone) and liposomal amphotericin B (AmBisome) in a rat model of pulmonary aspergillosis.Deposition: Fungizone or AmBisome was nebulized in healthy rats. At different times after nebulization, amphotericin B (AMB) was analyzed in lungs. Efficacy: Lungs of immunosuppressed rats were inoculated with 1.5x10E5 Aspergillus conidia. Treatment consisted of a single dose of nebulized Fungizone or AmBisome (nebulizer reservoir concentration 2 and 4 mg/ml, respectively) and was given 2 h, 24 h, 1, 2, 4 or 6 weeks before inoculation. Survival was monitored up to 12 days after inoculation.Pulmonary AMB concentrations directly after nebulization of Fungizone or AmBisome were 22.2 and 24.6 ug/g, respectively. AMB was cleared slowly from the lungs (t1/2íŸ > 20 days). Survival was prolonged after administration of either nebulized Fungizone or AmBisome in all prophylactic regimens as compared to untreated controls (p
2001

abstract No: 

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Full conference title: 

22nd International Congress of Chernotherapy (ICC)
    • ICC 22nd