Proteome maps of total cell, mitochondrial and secreted proteins of Aspergillus fum igatus.

Martin Vödisch 1*, Olaf Kniemeyer 1, Dirk W artenberg 1, Daniela Albrecht 1, Robert W inkler 2, Axel A. Brahhage 1

Author address: 

Leibniz Institute for Natural Product Research and Infection Biology (HKI) / Friedrich-Schiller-Universität Jena, Germany, 2Tecnologico de Monterrey, Departamento de Biotecnologia e Ing. de Alimentos, Mexico, [email protected]

Abstract: 

Aspergillus fumigatus is a ubiquitously distributed saprophytic mould. Upon inhalation, A. fumigatus spores germinate, undergo hyphal growth and spread in the lungs causing deadly invasive aspergillosis in immunocompromised patients. In comparison to other fungi, A. fumigatus possesses obviously higher stress tolerance and better mechanisms to adapt to the host environment, since this Aspergillus species is the prominent cause for such severe lung infections. As a basis for comparative proteomic studies, we established a reference proteome map. Using MALDI-TOF-MS/MS we identified 392 protein spots representing 344 proteins separated on 2D-gels. Proteins involved in primary metabolism, protein synthesis, cell cycle regulation, DNA processing and transcription, cellular organisation and stress response were most abundant. Since mitochondria are involved in many essential processes such as energy metabolism, cellular differentiation and cell death, we established a protocol for the isolation of mitochondria of A. fumigatus mycelium and constructed a 2-D reference-gel for this organelle as well. 136 proteins represented by 196 spots have been identified so far. To complete the analysis of the mycelial proteome, we analysed the secreted proteins (secretome) under in vitro conditions. In many cases, proteins secreted by pathogens play an important role for their virulence. For A. fumigatus only little information about secreted proteins and their contribution to virulence is available. About 80 different proteins, represented by 272 spots, were detected of which approximately 45% were predicted as secreted proteins by bioinformatic analysis. Proteases and proteins involved in cell wall metabolism were most abundant. *Student poster
2009

abstract No: 

24

Full conference title: 

6th International Aspergillus Meeting
    • Asperfest 6 (2009)