Proteome analysis of the response of Aspergillus fum igatus to voriconazole

Nansalmaa Amarsaikhan*, Olaf Kneimeyer , Zumrut Ogel

Author address: 

Department of Biotechnology, Middle East Technical University, Ankara, Turkey Department of Molecular and Applied Microbiology, Hans-Knoll Institute, Jena, Germany [email protected]

Abstract: 

Aspergillus fumigatus is the most important airborne fungal pathogen which can cause invasive aspergillosis in immunocompromised individuals, where the number of available antifungal drugs is limited due to its eukaryotic origin. Aspergillosis is usually treated by the administration of antifungal drugs, in most cases by the azole group drugs such as voriconazole. Recently, there has been increasing evidence for antifungal drug resistance in Aspergillus. For this reason, the research focus has shifted to investigating the key proteins involved in drug resistance. Commonly, it is known that development of antifungal drug resistance is associated with the upregulation of general stress response pathways. Thus, studies focusing on the transcriptional and proteomic profiles are of great importance to address these general mechanisms. In this study, we are studying the change of the protein expression level of A. fumigatus in response to voriconazole, an important azole group drug. As a result of this study, we will be able to compare the proteome data with transcriptome data released by Ferreira et al (2006).This study would be the first proteomic study directed to antifungal drug resistance mechanisms and would lead to the comprehensive evaluation of key proteins involved in this process. A greater understanding of azole resistance in A. fumigatus could lead to an improved antifungal therapy in future. *Student poster
2009

abstract No: 

33

Full conference title: 

6th International Aspergillus Meeting
    • Asperfest 6 (2009)