Protein Extraction from Fungi

In order to study proteins from yeasts and filamentous fungi, it is important to consider a number of basic features of the organisms. First, filamentous fungi undergo a growth cycle that includes differentiation and compartmentalization. In addition, both the filamentous fungi and yeasts will age during growth, and older cultures will undergo autolysis. As a result, particular proteins may only be associated with one part of the growth cycle, such as sporulation or autolysis, and this must be taken into account in determining growth conditions and sampling times.

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