Promoter-tagged restriction enzyme mediated insertion (PT-REMI) DNA mutagenesis was performed in the fungus, Aspergillus niger, using a plasmid containing a strong transcriptional promoter. Two DNA-tagged mutants were analyzed in detail. A white-spored mutant was shown to contain a plasmid insertion that disrupted a gene showing strong identity to the polyketide synthase wA gene of A. nidulans. A morphological mutant was shown to contain a plasmid insertion in the 5'-upstream region of a gene showing strong identity to cytochrome C oxidase subunit V, COXS. The insertion of the plasmid resulted in enhanced expression for the COXS RNA demonstrating that a combination of REMI with a promoter can be used to activate gene transcription.
Fungal Genet. Newsl. 46 (Supl):
Full conference title:
Fungal Genetics Conference 20th
- Fungal Genetics Conference 20th (1999)