Profiling of gene expression during polar growth of Aspergillus fumigatus

Ken Oda 1, Andrew Breakspear 2, Susan Cowden 1, Michelle Momany 1.

Author address: 

1Dept. of Plant Biology, Univ. of Georgia, Athens, USA., 2current address: Dept. of Plant Pathology, Univ. of Minnesota, St. Paul, USA, e-mail: [email protected]

Abstract: 

A. fumigatus is the most common airborne pathogen causing fatal mycoses in immunocompromised patients. Polarized growth is one of the critical factors for establishing fungal pathogenesis, but little is known about the genes involved in early polar growth and their regulation. The purpose of this study was to find polar growth related genes in A. fumigatus. A. fumigatus Af293 was cultured in complete medium and total RNA was extracted at set time points. DNA microarray experiments were performed comparing dormant cells (0hr) with isotropicly growing cells (4hr), isotropicly growing cells with cells showing emerging germtubes (6hr), and with more mature hyphae (8hr). Expression of selected genes was confirmed by RT-PCR. Comparison of altered genes in the three array experiments shows that the change of gene expression is most prominent at the switch from dormant to isotropic growth and that many genes show significant expression changes during early growth in A. fumigatus.
2009

abstract No: 

45

Full conference title: 

6th International Aspergillus Meeting
    • Asperfest 6 (2009)