Production of fermentable sugars from organosolv-treated cassava peels by lignocellulolytic organisms

Afolake A. Olanbiwoninu1, Sunday A. Odunfa2

Author address: 

1Ajayi Crowther University, Oyo Town, Oyo State, Nigeria, 2University of Ibadan, Ibadan, Oyo State, Nigeria

Abstract: 

Cassava peels are rich in lignocellulolytic materials which are not readily amenable to enzymatic hydrolysis; hence, the need for suitable pretreatment method that will support enzymatic hydrolysis. This study was designed to investigate lignocellulolytic organisms that support the bioconversion of organosolv pretreated cassava peels to fermentable sugars. Cassava peels were collected, microorganisms isolated, characterized and screened for lignocellulolytic enzymes production. Optimisation of enzymes production were determined. Organosolv pretreatment was carried out using methanol with varied concentration of catalyst (0.01 - 3M), reaction time (15 - 60 mins) and substrate size. Bioconversion was monitored using DNSA method, percentage peel hydrolysis was calculated. Fermentable sugars were quantified using gas chromatography.Pseudomonas fluorescens and Aspergillus terreus was isolated. P. fluorescens produces 2.8u/mL of crude enzymes optimally at 50oC and pH 8 while A. terreus produces 3.4u/mL optimally at 40oC, pH 6. Both isolates utilizes CMC and yeast extract best. Highest peel hydrolysis was 67% for P. fluorescens at 0.01M and 0.05M for A. terreus (94%). Highest concentration of fermentable sugar was produced by A. terreus (331.79mg/L glucose and 46.52mg/L xylose). P. fluorescens and A. terreus effectively supported the bioconversion of organosolv pretreated cassava peels to fermentable sugars

2015

abstract No: 

S20/18

Full conference title: 

Microbiology Society Annual Conference
    • MS 2015