Preparation of Solid Tissue for Aspergillus Galactomannan Antigen Detection by Platelia® (Biorad)

Author: 

Laboratoire de Parasitologie-Mycologie
Not Known

Abstract: 

AimPlatelia Aspergillus (Biorad) is a 1-stage immunoenzymatic sandwich microplate kit, for the detection of antigen galactomannan. FDA information about Platelia Aspergillus EIA can be found here. The technique was designed for use on human serum. However, it may also be possible to perform this method on solid tissues and organic solutions. Viscous solution and tissue specimens need to be pre-treated to achieve the extraction of the Aspergillus antigen and to get a homogeneous sample in solution.