Preparation of samples for scanning electron microscopy

Methods Mol Biol. 2007;369:449-66.

This chapter covers conventional methods for preparing biological specimens for examination in the scanning electron microscope (SEM). Techniques for handling cells grown in liquid culture, as well as on substrates such as culture dishes, slide culture chambers or agar, are discussed. These methods may be used to process most cultured organisms as well as whole botanical and zoological specimens.

- Preparation of Cell Suspensions

- Preparation of Cells Grown on Substrates

- Preparation of Tissues and Large Pieces of Biological Material

- Storage of Specimens Before the Completion of Drying

- Drying the Specimen (critical point dehydration)

- Mounting Dried Specimen on SEM Stub

- Coating Specimens With Heavy Metal

- Nonmechanical Coating of Specimens

- Storing SEM Specimen Stubs

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