PLEXID Ibis-Abbott technology as a single tool for the detection and typing of microbiological infections in immuno-compromised patients.

J. Le Goff, S. Mercier-Delarue, L. Feghoul, JL Pons, J. Menotti, A. Bergeron, F. Simon 1-4

Abstract: 

Introduction: The PLEXID technology (Abbott) multiplex PCR-combining mass spectrometry and Enables the detection and identification of a broad spectrum of pathogens in a single run.

Objectives: To compare the accuracy and the rate of infections detected by the PLEXID and routine analysis.

Methods: We Evaluated PLEXID 3 different assays; the Viral IC assay (73 RNA and DNA viruses' species), theBAC Detection assay (3,400 species of bacteria, 40 species of Candida) and the Broad Fungal assay (350 families of fungi).

Viral IC assay. We Analyzed 273 frozen samples from patients infected or not with adenovirus (Adv). Then we parallelized the routine and PLEXID in samples from 79 prospective hematopoietic stem cell and kidney transplant patients.

BAC detection assay. 48 peripheral blood and catheters and tested Were Compared To BACT Alert (bioMerieux) blood culture.

Fungal Broad assay . Broncho-Alveolar washings (BAL) and nasopharyngeal aspirates from 25 patients and tested Were Compared with fungal culture.

Results: Using Viral IC assay, Adv Was detected in 96.8% and in 78.0% of positive Adv frozen plasma and stool. 100% of species Were Correctly APPROBATION. Viral loads (R-gene ™ Adenovirus) Were correlated with PLEXID levels up to 6 log 10 copies / ml (r² = 0.67, p <10 -4 ). PLEXID detected in 66% of positive plasma Adv au moins un Reviews another virus. In the 79 prospective plasma correlated with results Were Those found in routine. CMV and EBV (plasma is PLEXID vs whole blood on routine) Were positive When the viral load Was Above 3.5 and 4.5 log respectivement. PLEXID APPROBATION 3 JC / BK, 2 HSV-1 and 1-parvo B19 infections in 6/79 patients from routine tests Whom Were not prescribed for viruses thesis.

BAC detection assay APPROBATION like Did the blood culture S heamolyticus in a catheter and a P entomophila / putida / aeruginosa mixture in a sepsis and a C. albicans DNA in a patient under antifungal therapy, the crop remaining negative. The C. albicans infection in liver, spleen and lung Was Then confirmed selon imaging results and a positive culture in a BAL The Following days. Other analyzes onto reference bacterial strains confirmed the perfect accuracy of PLEXID in bacterial identification. Finally, LAC assay APPROBATION from a frozen biopsy pulmonary year Actinomyces unrecognized by classical culture.

Fungal Broad assay APPROBATION Correctly all the samples. H capsulatum infection Was detected 19 days before the isolation culture. Candida species identification Was aussi perfectly in agreement with routine procedures.

Conclusions: PLEX-ID Showed a high sensitivity and accuracy in the identification of viruses, bacteria and fungus and enabled an early detection of pathogens or not amenable to recovery slowly in culture. The use of PLEX-ID as a single tool for fast screening viral infection in transplant patients Any prospective studies needs further Top goal opens a new deal in monitoring of These patients.

2012

Full conference title: 

Réunion Interdisciplinaire de Chimiothérapie Anti Infectieuse
    • RICAI 32nd (2012)