Phylogenetic Analysis of the Genus Aspergillus Based on RFLP of Repetitive and Single Copy Sequences.

D.Hoffman-Zacharska1*, K. Spalik2, P.Stephien1. 1 Dept. of Genetics, University of Warsaw, 2 Dept. of Plant

Author address: 

Systematic and Geography , University of Warsaw, Warsaw, Poland current address - Dept. of Genetics, Inst. of Psychiatry and Neurology, Warsaw, Poland

Abstract: 

Species of Aspergillus belong to the first fungal organisms that were cultivated on artificial media and studied for biochemical properties, they are also among the most common fungi in man's environment. Classical methods for systematic studies of this genus have been very successful and have provided a relatively good classification. However, morphological characters used for these studies probably do not reflect the real phylogenetic and evolutionary relationships between the taxa. In our work to determine the relationship among several species of the genus (39 strains representing 5 subspecies and 12 sections) we used restriction fragment length polymorphism (RFLP) of the unit coding three classes of rRNA - 5.8S, 18S) 26S. Using a plasmid containing the cloned rRNA unit from A.nidulans as a probe we found this method to be useful on the level of section. For more detailed analysis of strains representing section Nigri (22 strains) we additionally used RFLP analysis for a single copy gene - the gene coding glucoamylase from A.awamori.
1996

abstract No: 

NULL

Full conference title: 

3rd EUROPEAN CONFERENCE ON FUNGAL GENETICS
    • ECFG 3rd (1996)