Penetration of amphotericin B into the cornea following systemic with amphotericin B and its lipid preparations in a rabbit model

D. Goldblum, K. Rohrer, B. E. Frueh, R. Theurillat, W. Thormann, S. Zimmerli, Bern, CH


Objectives: In fungal keratitis antifungal drugs are commonly applied topically as well systemically for fear of threatening exogenous endophthalmitis. Amphotericin B (AmB) various lipid formulations of AmB are available for the treatment of fungal infections Their fungicidal activity against a broad range of pathogenic fungi makes these drugs choice for the empirical therapy of keratomycoses. Little is known, however, about their penetration into the cornea following systemic treatment. The corneal concentration amphotericin B following 7 daily doses of parenteral AmB lipid complex (ABLC) or liposomal (L-AmB) was compared to that of AmB deoxycholate (D-AmB) in a rabbit model. Methods: After induction of unilateral uveitis by intravitreal injection of endotoxin, rabbits per treatment) were given daily doses of D-AmB (1 mg/kg) ABLC (5 mg/kg) or L-AmB by central venous catheter on 7 consecutive days. AmB concentrations were determined high-pressure liquid chromatography in corneae collected at autopsy 24 h after the 7th dose. were analysed using the Mann-Whitney U-test. Results: After 7 days of treatment, mean corneal concentrations of AmB in the inflamed were highest after treatment with L-AmB (2.38 ± 1.47 µg/g) compared to ABLC (

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11th European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 11th (2001)