PCR for early diagnosis of invasive fungal infections in immunocompromised patients: Where are we?

L. Klingspor*

Author address: 

(Stockholm, SE)


Invasive Fungal infections (IFI) are important causes of morbidity and mortality in immune-compromised patients. Diagnosis relies on culturebased methods, which lack sensitivity and delay diagnosis. PCR for the diagnosis of IFI offers an attractive method for early diagnosis. However, a lack of standardization of fungal PCR assays has limited its acceptance as a diagnostic tool. There is currently no consensus on the optimal blood fraction from which to isolate Candida DNA. A PCR detection limit of 3 mL) should be be efficiently lysed before bead beating to disrupt the fungal cell and use of an internal control PCR to exclude false negativity. DNA should be eluted in volumes of

abstract No: 


Full conference title: 

22nd European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 22nd (2012)