Patients With Allergic Bronchopulmonary Aspergillosis and Atopy

JS McClellan*, ST Roodman*. AJ White?. TA Chntilla f, AP Knutsen*

Author address: 

*St Louis University Health Sciences Center, St Louis, MO twashington University, St Louis, MO

Abstract: 

OBJECTIVE: Previously, we reported that induction of CD23 expression on B cells was more sensitive to IL-4 stimulation in expressed stimulation in ABPA patients compared to atopic and non-atopic individuals JACI 103:S16, 1999). Chatila et al reported a novel interleukin-4 receptor alpha chain (IL-4Ra) heterozygous mutation in 65% of atopic and 16% of nonatopic individuals. The presence of this mutant allele has been associated with an increased B cell IL-4 sensitivity. In this study, mutations of IL-4Ra chain were examined as the mechanism for increased IL-4 sensitivity. METHODS: We studied 4 ABPA, 7 atopic, and 6 non-atopic patients. Peripheral MNC isolated from whole blood were stimulated for 48 hours with varying concentrations of rIL-4 (OS-50ng).Both the percentage and the total amount of CD23 expression of CD20+ B cells were quantified by flow cytometry with calibrated standardized PE-conjugated beads. Mutations of interleukin-4 receptor a (IL-4Ra) R576 in lymphocytes were analyzed for the presence or absence of the R576 glutamine to arginine mutation analyzed by single-strand conformation polymorphism (SSCP) on EBV-transformed B cells. RESULTS: The expression of CD23 per CD20+ B cell prior to stimulation was significantly higher in atopic and ABPA patients compared to non-atopic controls, 17, I76 and 14.4770 CD23+ molecules/cell versus 11,688 CD23+molecules/cell (p
2000

abstract No: 

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Full conference title: 

2000 American Academy of Allergy, Asthma, and Immunology Annual Meeting
    • AAAAI 2000 (56th)