Objective: Fungi producing otomycosis vary by geographic areas. Among the yeasts, Candida albicans and C. parapsilosis are the most common species isolated. Among the molds, Aspergillus niger is predominant, followed by other species such as A. fumigatus and A. flavus. Other molds are involved occasionally in otomycosis. We present the first case of otomycosis caused by Chrysosporium tropicum. Case report: A 30-year-old non-diabetic male, welder by profession, living in the countryside, presented history of chronic external otitis in the right ear, for 2 months. He complained of mild pain in the ear at the beginning, hypoacusia by plugging itching during 6 days. OnExclusion criteria. Patients who had received antibiotics, antifungals antiinflammatory or 3 months before the study. Patients who had received periodontal treatment 3 months prior to the experience. Along with conventional systemic history, they will conduct a survey. Subgingival samples were collected to Eppendorf tubes were placed in saline solution were extended the same time each study site to color with the technique of Gram and Giemsa. The samples were grown to study CHROMagar Candida spp. under aerobic conditions micromorphology was observed in 1% milk agar-Tween 80 (28), urease production and carbohydrate assimilation profile by commercial systems 32D Api ID,?(BioMe’rieux France). In addition, species that develop green on the chromogenic media were subjected to assimilation of xylose, micromorphology on Staib medium (29), growth at 45 to confirm the species and DNA amplification by PCR with primers or primers specific molecular level to differentiate C. dubliniensis from other Candida spp. Results: Patients taking drugs for treatment had a higher incidence of chronic periodontitis in the control group. Only patients who took these drugs were isolated non-albicans Candida spp.: C. tropicalis, C. krusei. Conclusions: Gingival Fluids circulating hormones increase the carrying of Candida spp. in oral cavity.
Full conference title:
Trends in Medical Mycology, 5th
- TIMM 5th (2013)