NpkA, a cdc2-related kinase from Aspergillus nidulans, interacts with the UvsBATR kinase. .

Fagundes, Mrvzk1, Savoldi, M1, Lima, JF1, Malavazi, I1, Larson, RE2, Goldman, MH3; Goldman, GH1.

Author address: 

1Faculdade de Ciëncias Farmacëuticas de Ribeirão Preto; 2Faculdade de Medicina de Ribeirão Preto; 3 Faculdade de Filosofia Ciëncias e Letras de Ribeirão Preto,USP, Brazil

Abstract: 

The DNA damage response is a protective mechanism that ensures the maintenance of genomic integrity. We have been using Aspergillus nidulans as a model system to characterize the DNA damage response caused by the anti-topoisomerase I drug, camptothecin (CPT). We report the molecular characterization of a p34Cdc2-related gene, npkA, that is transcriptionally induced by CPT and other DNA-damaging agents, and its induction in the presence of CPT is dependent on the uvsBATR gene. The npkA deleted strain can partially suppress HU-sensitivity caused by the uvsBATR and uvsD153ATRIP checkpoint mutations. We demonstrated that uvsBATR gene is involved in DNA replication and the intra-S-phase checkpoints, and that the npkA deleted strain can suppress its intra-S-phase checkpoint deficiency. There is a defect in both the intra-S-phase and DNA replication checkpoints due to the npkA inactivation when DNA replication is slowed at 6 mM HU. Our results suggest that npkA gene plays a role in cell cycle progression during S phase as well as in a DNA-damage signal transduction pathway in A. nidulans. Financial Support: FAPESP e CNPq
2005

abstract No: 

568

Full conference title: 

23rd Fungal Genetics Conference
    • Fungal Genetics Conference 23rd (2002)