Novel Fungal Phytase Genes and Enzyme Activities

David B. Mitchell, Kurt Vogel, Luis Pasamontes and Adolphus P.G.M.

Author address: 

van Loon. Biotechnology Section, Vitamins and Fine Chemicals Division, F. Hoffmann-La Roche Ltd, CH-4070 Basel, Switzerland

Abstract: 

We isolated and characterised the complete genes encoding novel fungal phytases from Aspergillus terreus and Myceliophthora thermophila and PCR fragments of the phytase genes from 4 other fungi. The encoded proteins show 48-60% identity compared to the phytase of Aspergillus niger and have 21-29% identity when compared with known acid phosphatases. The phytases form a separate subclass of the histidine acid phosphatase family. Phytase genes of additional fungal species were identified by cross-hybridization experiments. Interestingly, no cross-hybridization with the Aspergillus niger phytase gene was seen. The phytases of Aspergillus terreus and Myceliophthora thermophila showed novel enzyme activity profiles and have a higher preference for phytic acid as substrate compared to 4-nitrophenyl phosphate, than the A. niger phytase. Further characterization of the genes and enzymes is underway.
1996

abstract No: 

NULL

Full conference title: 

3rd EUROPEAN CONFERENCE ON FUNGAL GENETICS
    • ECFG 3rd (1996)