Multidrug resistance can be caused by increased ATP-dependent efflux of toxic compounds from cytoplasm and plasma membrane mediated by membrane-bound ATP-dependent transporters of the ABC (ATP-Binding Cassette) superfamily. As a preliminary step to characterizing genes encoding ABC-proteins that confer multiple drug resistance in Aspergillus nidulans, we are using a PCR-based approach. Five degenerated primers designed based on the coding regions of the ATP-binding cassette were used to identify such genes. DNA fragments in the expected size were amplified. These fragments were cloned and several subclones were sequenced. Sequence and Southern blot analysis showed that these fragments correspond to four different genes encoding ABC-transporters (named abcA-D, respectively). Northern blot analysis showed that the abcD gene transcript size is around 6.0-kb and this gene is induced about five-fold by miconazole, three-fold by ethidium bromide, and two-fold by camptothecin. Financial support: FAPESP, CNPq, and CAPES, Brazil, and ICGEB-UNIDO.
Fungal Genet. Newsl. 46 (Supl):
Full conference title:
Fungal Genetics Conference 20th
- Fungal Genetics Conference 20th (1999)