THE NON-RIBOSOMAL PEPTIDE SYNTHETASE, PES1, IN ASPERGILLUS FUMIGATUS MEDIATES IN VITRO ANTIFUNGAL DRUG RESISTANCE AND CONTRIBUTES TO GROWTH ABILITY UNDER CONDITIONS OF SIDEROPHORE DEFICIENCY

L. Gallagher1*, C. Jöchl1, K. Kavanagh1, H. Haas2, S. Doyle1

Author address: 

1Dept. of Biology, National University of Maynooth, Ireland 2Division of Molecular Biology, Innsbruck Medical University, Austria

Abstract: 

Purpose: Aspergillus fumigatus produces metabolites through a non-ribosomal biosynthetic mechanism, including many of unknown function as well as iron-chelating molecules known as siderophores. Limited information is available with respect to non-ribosomal peptide function and to date, potential interactions between non-ribosomal peptide products has received little attention. Methods: An A. fumigatus mutant deficient in the NRPS, pes1 (18 kb)(AfuA_1g10380/NRPS1), was generated by removing the first 3 kb of the open reading frame, including the promoter region and replacing it with a pyrithiamine resistance marker (ptrA) using the bi-partite gene replacement method (Nielsen et al. 2006). Results: Compared to wild-type, A. fumigatus 916;pes1 showed reduced growth in the presence of two front line chemotherapeutic agents, voriconazole (p
2010

abstract No: 

99

Full conference title: 

4th Advances Against Aspergillosis
    • AAA 4th (2010)